Abundance of fishes of the main channels of the upper Mississippi River sys
tem and of other large North American rivers is largely unknown because his
toric sampling methods have been inadequate. We used a bottom trawl to esti
mate spatial and temporal patterns in abundance in the navigation channels
of Pool 26 of the Mississippi River and the lower Illinois River. Total bio
mass density averaged 21 and 29 kgha(1) in the navigation channels of Pool
26 and the lower Illinois River, respectively. We identified spatial and te
mporal patterns in catches of key species using a generalized linear model
based on the negative binomial distribution. Some species, including shovel
nose sturgeon (Scaphirhynchus platorynchus), are persistent residents of th
e main channel. Multiple-season residents, including freshwater drum (Aplod
inotus grunniens), rely heavily on the main channel during most of the year
but leave it briefly, for example to seek thermal refugia in backwaters du
ring winters. We suggest revision of the prevailing notion that main channe
ls of large temperate rivers serve mainly as corridors for movement among o
ther habitat types.