Levels of ACC and related compounds in exudate and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria

It was previously proposed that plant growth-promoting bacteria that posses s 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase could utilize ACC t hat is present in the exudate of germinating canola seeds. The uptake and c leavage of ACC by these bacteria would lower the level of ACC, and thus eth ylene within the plant, and reduce the extent of its inhibition on root elo ngation. To test part of the above mentioned model, ACC levels were monitor ed in canola seed tissues and exudate during germination. Lower amounts of ACC were present in the exudate and tissues of seeds treated with the plant growth-promoting bacterium Enterobacter cloacae CAL3, than in control seed s treated with MgSO4. The ACC-related compounds, alpha- and gamma -aminobut yric acids, both known to stimulate ethylene production, were also measured in the canola seed exudate and tissues. Approximately the same levels of a lpha -aminobutyric acid were present in the exudates of the bacterium-treat ed seeds and the control seeds, but the amount of alpha -aminobutyric acid was lower in the tissues of the bacterium-treated seeds than in the control seeds. Smaller quantities of gamma -aminobutyric acid were seen in both th e exudate and tissues of the E. cloacae CAL3-treated seeds than in the cont rol seeds.