Genetic transformation of calli from maize and regeneration of herbicide-resistant plantlets

The herbicide-resistant gene als of Arabidopsis thaliana has been transferr ed into embryonic calli of maize by microprojectile bombardment. We have ob tained chlorsulfuron-resistant calli and regenerated plantlets through sele ction by herbicide chlorsulfuron, The results of PCR analysis and Southern blotting indicate that gene als has been transferred into some plantlets, T he test of spraying chlorsulfuron indicated that the transgenic plantlets h ad favorable herbicide resistant trait. The purpose of the research was to obtain chlorsulfuron-resistant transgenic maize and hope that this hind of high efficient herbicide could be widely used in rotation soil of wheat and maize. In addition, through spraying herbicide, we could eliminate the hyb rid plants and thereby increase the purity of F1 seeds.