Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction

Objective A new quantitative polymerase chain reaction (real-time PCR) was designed to detect Toxoplasma DNA in human body fluid samples. Methods Real-time fluorescence detection of amplification product formation on the basis of the TaqMan-System was established with Toxoplasma 18S rDNA as a target gene. Results The method provides a high sensitivity comparable to conventional n ested PCR procedures and generates quantitative data when detecting toxopla smic DNA in human blood, cerebrospinal or amniotic fluid. Moreover, data we re obtained investigating blood samples from an immunocompromised patient w ith reactivated toxoplasmosis after allogeneic bone marrow transplantation. monitoring the therapeutic effect. Conclusions The potential application of this method to detect Toxoplasma,l n DNA in body fluids and to follow the development of parasitemia under the rapy could be demonstrated.