Comparative analysis of the genetic structure and chromosomal location of the murine MyD118 (GADD45 beta) gene

The MyD118 (Gadd45 beta) protein is a member of a family of structurally re lated proteins, including Gadd45 (Gadd45 alpha) and CR6 (Gadd45 gamma), tha t have critical roles in regulating growth arrest and apoptosis, The MyD118 and other members of its family display distinct patterns of expression in response to stimuli that induce differentiation, growth arrest, or apoptos is. Species-blot analysis showed that MyD118 is an evolutionarily conserved gene, and comparative sequence analysis showed that MyD118 has a gene stru cture similar to that of other members of its gene family. Comparison of pu tative transcription factor-binding sites found in sequences of this gene f amily provides evidence that p53 is involved in regulating the expression o f MyD118 and that NF-kappaB may play a role in differential expression of M yD118 and Gadd45(Gadd45a). fluorescence in situ hybridization localized the MyD118 gene to mouse chromosome band 10B5.3, correcting a previous assignm ent to mouse chromosome 9.