Cerebellar astrocytes coexpress different purinoceptors: Cross-talk between several transduction mechanisms

We characterized the purinergic receptors present in individual type-1 astr ocytes from rat cerebellum by using single cell microfluorimetry with fura- 2. We found that individual astrocytes coexpressed several P2Y purinoceptor s coupled to phospholipase C activation through different G proteins. P2Y(1 ) and P2Y(2) or P2Y(4) purinoceptors were responsible for the ATP, 2MeSATP, and UTP calcium responses observed in all tested cell. A subpopulation of astrocytes also coexpressed a specific pyrimidine receptor, which was respo nsible for UDP calcium responses recorded only from a subpopulation of astr ocytes. Type-1 astrocytes also present A(2B) adenosine receptors positively coupled to adenylate cyclase. There was a positive interaction between A(2 B) adenosine receptors and P2Y(1) or P2Y(2) or P2Y(4) purinoceptors express ed in the same astrocyte. We also found that the stimulation of astrocytes with nanomolar concentrations of Ap(5)A produced the activation of extracel lular signal regulated kinases (ERK), which could be responsible for the po tentiation of ATP calcium responses elicited by the dinucleotide, Drug Dev. Res. 52:114-121, 2001. (C) 2001 Wiley-Liss, Inc.