Intact cultured cells of Nicotiana tabacum BY2 cells exhibited the electroc
hemical reduction of I, l-benzoquinone (BQ), 2,6-dichloroindophenol (DCIP),
hexacyanoferrate (ferricyanide). This was detected by amperometry with fix
ed potential (400 mV vs. Ag/AgCl) using a film-coated phi 1.6 mm platinum d
isk electrode as a working electrode. The electrochemical activities of BQ,
DCIP, and ferricyanide mixed with BY2 cell were changed after the addition
of various stress compounds such as elicitors, plant hormones, metabolic a
nd electron transport inhibitors. Different stress compounds gave di differ
ent electrochemical patterns probably due to the different positions of ele
ctron accepters affected by these bio-materials. In addition, we observed t
he generation of hydrogen peroxide in BY2 cells treated with various stress
compounds, The pattern of chemiluminescence is useful for the determinatio
n of the intensity of plant stress responses. High accumulation of hydrogen
peroxide was observed in the BY2 cells treated with elicitors (salicylic a
cid and ethylene glycol chitin) and plant hormone (2,3-D). A relationship b
etween electrochemical patterns and stress compounds were also discussed.