Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate

Authors
Lomolino, G Lante, A Crapisi, A Spettoli, P Curioni, A
Citation
G. Lomolino et al., Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate, ELECTROPHOR, 22(6), 2001, pp. 1021-1023
Citations number
13
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
01730835 → ACNP
Volume
22
Issue
6
Year of publication
2001
Pages
1021 - 1023
Database
ISI
SICI code
0173-0835(200104)22:6<1021:DOSCCA>2.0.ZU;2-H
Abstract
A simple method for the visualisation of wine yeast esterase (carboxylester ase EC 3.1.1.1)activity on electrophoretic gels was developed, using the fl uorescent substrate fluorescein diacetate. The zymogram system allows a sen sitive detection of esterase bands in only 5 min of incubation of both nati ve and sodium dodecyl sulfate gels.