Structural and serological studies on Hafnia alvei O-specific polysaccharide of alpha-D-mannan type isolated from the lipopolysaccharide of strain PCM 1223

Citation
E. Katzenellenbogen et al., Structural and serological studies on Hafnia alvei O-specific polysaccharide of alpha-D-mannan type isolated from the lipopolysaccharide of strain PCM 1223, FEMS IM MED, 30(3), 2001, pp. 223-227
Citations number
22
Categorie Soggetti
Immunology
Journal title
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
ISSN journal
09288244 → ACNP
Volume
30
Issue
3
Year of publication
2001
Pages
223 - 227
Database
ISI
SICI code
0928-8244(200104)30:3<223:SASSOH>2.0.ZU;2-I
Abstract
On the basis of chemical and methylation analyses, one- and two-dimensional H-1- and C-13-NMR spectroscopy, including COSY, TOCSY. NOESY and H-1, C-13 HSQC experiments. a neutral O-specific polysaccharide isolated from Hafnia alvei Strain PCM 1223 lipopolysaccharide (LPS) was found to be an a-mannan composed of pentasaccharide repeating units having the following structure : --> 3) - alpha - D - Manp-(1 --> 3) - alpha - D - Manp-(1 --> 2) - alpha - D - Manp-(1 --> 2) - alpha - D - Manp-(1 --> 2) - alpha - D - Manp-(1 --> Immunoblotting showed a strong cross-reactivity between anti-H. alvei PCM 1 223 serum and LPSs of Escherichia coli O9 and Klebsiella pneumoniae O3. The serological relationship of the LPSs of these bacteria is due to the struc tural identity of their O-specific polysaccharides, though the LPSs differ in their core regions. (C) 2001 Federation of European Microbiological Soci eties. Published by Elsevier Science B.V. All rights reserved.