DYS STR analysis with epithelial cells in a rape case

We report here the application of Y-chromosomal DNA analysis in a rape care , which occurred in Stuttgart, Germany. Microscopic examination of the vict im's vaginal swabs and hel underwear showed no sperm cells. DNA was extract ed from vaginal and epithelial cells and analysed with the autosomal system s SE33. THO1 (singleplex) and with the multiplex Profiler Plus (Applied Bio systems, Foster City, USA). The results of these autosomal STR analysis gav e no hint at a mixed sample and failed to identify a male profile. DYS STR analysis with the systems DYS391, DYS392, DYS393, DYS19 and DYS389 I/II sho wed the same characteristic features as the suspect. We used this incomplet e haplotype to search in the Y-STR Haplotype Reference Database via Interne t. In a Caucasian population sample of 3589 minimal haplotypes we found 71 matches. The suspect confessed the dime and was Finally condemned to 4 year s imprisonment. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.