Superoxide dismutase biosensors working in non-aqueous solvent

Enzymatic electrodes based on superoxide dismutase enzyme were developed. U sing the superoxide dismutase enzyme sensor assembled according to the clas sical model, poor results were obtained, Results were improved by adopting a new way of assembling the biosensor using a cellulose triacetate layer in which thp SOB enzyme is entrapped and sandwiched between two gas-permeable membranes, or using a kappa-carrageenan el layer entrapping the, enzyme, s andwiched between an external gas permeable membrane and an internal cellul ose acetate membrane, coupled in each case to the oxygen amperometric trans ducer. Results obtained by applying the newly developed biosensor to assayi ng hydrophobic compounds showing radical scavengring properties, operating in dimethylsulfoxide, were also satisfactory.