Cj. Bean et al., Analysis of a malsegregating mouse Y chromosome: evidence that the earliest cleavage divisions of the mammalian embryo are non-disjunction-prone, HUM MOL GEN, 10(9), 2001, pp. 963-972
Despite the clinical importance of human aneuploidy, we know little of the
causes of mammalian non-disjunction, In part, this reflects the fact that,
unlike lower organisms, segregation 'impaired' chromosomes are virtually no
n-existent in mammals. To address this issue, we have studied the mouse Y c
hromosome on the BALB/cWt ('Wt') inbred background, a system in which loss
of the Y chromosome in gonadal tissue has been linked to hermaphroditism. O
ur results indicate that the Wt Y chromosome is stably transmitted during m
eiotic cell divisions, but non-disjoins at an extremely high frequency in m
itosis. Surprisingly, the nondisjunction events are largely restricted to t
he earliest cleavage divisions, indicating that there is a temporal 'window
' during which the Wt Y chromosome is susceptible to non-disjunction. The n
on-disjunction phenotype has both cis and trans components: the Wt Y chromo
some malsegregates on a variety of genetic backgrounds, demonstrating an in
trinsic defect; however, the incidence of non-disjunction is significantly
influenced by strain background, indicating the existence of modifying loci
and thus providing evidence for a genetic effect on mammalian nondisjuncti
on, These studies suggest that the earliest cell divisions in mammals are n
on-disjunction-prone, an interpretation which provides an explanation for t
he high rate of chromosome mosaicism observed in studies of in vitro fertil
ization (IVF)-derived human preimplantation embryos. Further, our observati
ons raise the possibility that the IVF setting adversely affects chromosome
segregation and suggest that genetic quality be an important consideration
in any attempt to improve or modify in vitro procedures for use on human e
ggs and embryos.