A. Almagro et al., Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii, J BACT, 183(10), 2001, pp. 3251-3255
Two genes encoding Na+-ATPases from Debaryomyces hansenii were cloned and s
equenced. The genes, designated ENA1 from D. hansenii (DhENA1) and DhENA2,
exhibited high homology with the corresponding genes from Schwanniomyces oc
cidentalis. DhENA1 was expressed in the presence of high Na+ concentrations
, while the expression of DhENA2 also required high pH. A mutant of Sacchar
omyces cerevisiae lacking the Na+ efflux systems and sensitive to Na+, when
transformed with DhENA1 or DhENA2, recovered Na+ tolerance and also the ab
ility to extrude Na+.