Osmoregulated periplasmic glucan synthesis is required for Erwinia chrysanthemi pathogenicity

Erwinia chrysathemi is a phytopathogenic enterobacterium causing soft rot d isease in a wide range of plants, Osmoregulated periplasmic glucans (OPGs) are intrinsic components of the gram-negative bacterial envelope, We cloned the opgGH operon of E, chrysanthemi, encoding proteins involved in the glu cose backbone synthesis of OPGs, by complementation of the homologous locus mdoGH of Escherichia coli. OpgG and OpgH shown a high level of similarity with MdoG and MdoH, respectively, and mutations in the opgG or opgH gene ab olish OPG synthesis. The opg mutants exhibit a pleiotropic phenotype. inclu ding overproduction of exopolysaccharides, reduced motility, bile salt hype rsensitivity,, reduced protease, cellulase, and pectate lyase production, a nd complete toss of virulence. Coinoculation experiments support the conclu sion that OPGs present in the periplasmic spare of the bacteria are necessa ry for growth in the plant host.