Clean adiabatic TOCSYs

Various adiabatic mixing schemes are explored using numerical simulations w ith the aim of finding suitable mixing sequences for fast rotating samples with relatively wide resonances and poorly resolved J-couplings, such as pr oteins or resin-bound products of solid-phase synthesis. It is shown both e xperimentally and by simulations that WURST-2 mixing scheme provides the be st sensitivity and the lowest interference from cross-relaxation effects in the spin diffusion limit. For medium-sized molecules, WURST-1, constant ad iabaticity Gaussian and hyperbolic secant are the possible alternatives. Co pyright (C) 2001 John Wiley & Sons, Ltd.