Construction of a high-resolution RH map of the human 2q35 region on TNG panel and comparison with a physical map of the porcine homologous region 15q25

This article describes the construction of a high-resolution radiation hybr id map of Hsap 2q35 by using the TNG RH panel generated by irradiation with 50,000 rods. We were able to build a framework map of 1300 cR(50,000) incl uding 34 markers ordered with odds higher than 1000:1. The comprehensive ma p includes 77 loci and describes a region of 3 Mb around the SLC11A1 gene. Because of the very small size of the fragments retained and a reduced rete ntion frequency, it was difficult to build a high-resolution multi-point ma p of this region by using the TNG RH panel. Nevertheless, this study confir med the very high potential of this RH panel for constructing a human, high -resolution physical map (2.3 kb/cR(50,000)). Moreover, human ESTs from Hsa p 2q35 were hybridized with porcine BAC contigs to establish a porcine tran script map of the homologous region Sscr 15q25 (greater than 2.5 Mb). We id entified 17 new genes in this porcine chromosomal region. We were able to c ompare the location of 26 genes mapped in both species. The gene order was similar except for two possible minor discrepancies in the Desmin sub-regio n, suggesting the existence of a porcine micro-region between TNP1 and IL8R B with an unknown origin.