Isolation of novel human fetal brain cDNAs mapped to human chromosome bands, 1q25 and 8q24.1

We isolated chromosome band-specific human fetal brain cDNAs by the microdi ssection mediated cDNA capture method, and localized these cDNA using in si tu hybridization histochemistry with developing rat brain sections, Uni-Amp cDNAs were prepared fi om an 18-week old human fetal bl ain, and hybridize d to human metaphase chromosomes, Eight Uni-Amp cDNAs, hybridized to chromo some band 1q25 or 8q24.1, were recovered by microdissection and PCR amplifi cation with Uni-Amp primers, Among these cDNAs, two novel genes (FB113 of 8 q24.1 and FB134 of 1q25) showed a temporospatially interesting expression p attern in the developing rat brains, The expression of FB113 was under dyna mic regulation in the developing granule cells of cerebellum and dentate gy rus, FB134 showed a nervous tissue specific expression pattern and an exclu sively prominent expression in the developing presubiculum and parasubiculu m, By the fluorescence in situ hybridization using human genomic DNAs, FB11 3 and FB134 were mapped back to the human chromosome bands 8q24.1 and 1q25, respectively. These results indicate that combined application of the micr odissection mediated cDNA capture method and irt situ hybridization histoch emistry can be used for the isolation of chromosomal band-specific genes re lated to brain development or human genetic diseases.s