Quantitative determination of noncovalently bound acridinium in protein conjugates by liquid chromatography/electrospray ion trap mass spectrometry

Authors
Adamczyk, M Gebler, JC Shreder, K Wu, J
Citation
M. Adamczyk et al., Quantitative determination of noncovalently bound acridinium in protein conjugates by liquid chromatography/electrospray ion trap mass spectrometry, RAP C MASS, 15(9), 2001, pp. 670-674
Citations number
27
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
09514198 → ACNP
Volume
15
Issue
9
Year of publication
2001
Pages
670 - 674
Database
ISI
SICI code
0951-4198(2001)15:9<670:QDONBA>2.0.ZU;2-U
Abstract
A sensitive and robust liquid chromatography/electrospray ion trap mass spe ctrometry (LC/MS/MS) method has been developed for the quantitative determi nation of noncovalently bound acridinium free acid in protein-acridinium co njugates. The lower level of quantitation (LOQ) for acridinium free acid wa s determined to be 0.6 ng. The assay was validated with a linear concentrat ion range of 0.6-60 ng. The method requires minimum sample handling and is specific, reproducible, and provides a new aspect for protein-acridinium co njugate characterization. Copyright (C) 2001 John Wiley & Sons, Ltd.