Fluorescence quenching study on the interaction of bases of nucleic acid with electron-accepting sensitizer and serum albumin containing electron-rich tryptophan residue

Citation
Sp. Wu et al., Fluorescence quenching study on the interaction of bases of nucleic acid with electron-accepting sensitizer and serum albumin containing electron-rich tryptophan residue, RES CHEM IN, 26(7-8), 2000, pp. 727-737
Citations number
18
Categorie Soggetti
Chemistry
Journal title
RESEARCH ON CHEMICAL INTERMEDIATES
ISSN journal
09226168 → ACNP
Volume
26
Issue
7-8
Year of publication
2000
Pages
727 - 737
Database
ISI
SICI code
0922-6168(2000)26:7-8<727:FQSOTI>2.0.ZU;2-X
Abstract
Static and dynamic fluorescence quenchings of electron-accepting sensitizer s including positive charged heterocyclics and neutral cyanoaromatics by ba ses of nucleic acid (NB) have been investigated. It was found that NE could act as effective electron donors to quench the fluorescence of electron-ac cepting sensitizers. The quenchings by diffusion-controlled rate coincide w ell with the static and dynamic Stern-Volmer correlation. On the other hand , the diffusion-controlled fluorescence quenchings of the tryptophan (TRP) residue in the protein enzymes, HSA and BSA, by electron-accepting ND revea l that photochemical dual-damage of protein enzyme and thymine (THM) occur upon u.v.-irradiation, which is characteristic of excitation wavelength-dep endence. Therefore, the results illustrate that the lesion interactions of NE with electron-deficient sensitizers or electron-rich TRP fluorophore-con taining protein enzymes originate mainly from PET-initiated processes in bo th cases.