Peptidoglycan from sterile human spleen induces T-cell proliferation and inflammatory mediators in rheumatoid arthritis patients and healthy subjects

Objectives. Peptidoglycan (PG), a component of Gram-positive bacteria, may be involved in rheumatoid arthritis (RA) because of its ability to induce p roduction of proinflammatory cytokines. to induce arthritis in rodents, and its presence in antigen-presenting cells in RA joints. Methods. In the present study, physiologically relevant PG was able to indu ce T-cell proliferation in peripheral blood and synovial fluid samples of R A patients, but the magnitude of the response did not differ from that of c ells from healthy subjects. In addition, production of cytokines associated with RA (interleukins (IL)-1 beta, IL-6, IL-8, IL-10, IL-12 and tumour nec rosis factor alpha) and of the matrix metalloproteinase. gelatinase B (MMP- 9). was induced in blood and synovial fluid cultures of RA patients. Conclusion. The fact that PG, which can be found in synovial tissues of RA patients is able to induce the production of inflammatory mediators support s the hypothesis that PG plays a role in the pathogenesis of RA by influenc ing the inflammatory microenvironment of the joint.