The complete genome of Enterovirus 71 China strain

Five overlapping clones covering the full genome of Enterovirus 71 China st rain SHZH98 were obtained and then the sequences were determined by the cha in termination method. It showed that the full length of EV71 SHZH98 genome (not including Poly A tail) is 7408 bp. There are some diversities on the lengths and sequences of 5 ' UTR and 3 ' UTR between SHZH98 and the other E V71 strains. In P1 capsid region, which is closely associated with viral im munogenicity, EV71 strain SHZH98 shares the highest homology with Taiwan st rains; but in P2 and P3 non-structural gene regions there are higher identi ties with Coxsakievirus A16 and EV71 strains MS, BrCr than with Taiwan stra ins. Phylogenetic tree constructed by structural gene region indicates that China strain SHZH98 has a closer relationship with Taiwan strains, however , in the non-coding region it has a closer relationship with Coxsakievirus A16, EV71 strains MS and BrCr. EV71 China strain was analyzed at the molecu lar level. The results will contribute to the basic study on enteroviruses and the EV71 prevention in China.