Due to a shortage of organs for transplantation, many centres use marginal
grafts to increase their donor pool. As kidneys from non-heart-beating dono
rs (NHBD) have sustained initial ischaemic damage, their viability is diffi
cult to predict. Hypothermic pulsatile perfusion has not only been used to
improve the condition of such grafts, but also allows viability assessment.
Suitable systems are becoming more readily available, but they are expensi
ve. We have used existing dialysis equipment with modified sterilised inser
ts to create a pulsatile hypothermic perfusion system. With this system, 41
NHBD kidneys were perfused for up to 8 h; their intravascular renal resist
ance (IRVR), flow characteristics as well as glutathione S transferase (GST
) measurements were performed to assess viability. This hypothermic pulsati
le perfusion system is now an integral component of our NHBD programme.