T. Suzuki et al., Bafilomycin A(1) inhibits rhinovirus infection in human airway epithelium:effects on endosome and ICAM-1, AM J P-LUNG, 280(6), 2001, pp. L1115-L1127
Citations number
47
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
To examine the effects of bafilomycin A(1), a blocker of vacuolar H+-ATPase
, on rhinovirus (RV) infection in the airway epithelium, primary cultures o
f human tracheal epithelial cells were infected with RV14. Viral infection
was confirmed by showing that viral RNA in the infected cells and the viral
titers in the supernatants of infected cells increased with time. RV14 inf
ection upregulated the production of cytokines and mRNA of intercellular ad
hesion molecule (ICAM)-1 in epithelial cells. Bafilomycin A(1) reduced the
viral titers of RV14 and inhibited the production of cytokines and ICAM-1 b
efore and after RV14 infection. Bafilomycin A(1) reduced susceptibility of
epithelial cells to RV14 infection. RV14 increased activated nuclear factor
-kappaB in the cells, and bafilomycin A(1) reduced the activated nuclear fa
ctor-kappaB. Bafilomycin A(1) decreased the number of acidic endosomes in t
he epithelial cells. These results suggest that bafilomycin A(1) may inhibi
t infection by RV14 by not only blocking RV RNA entry into the endosomes bu
t also reducing ICAM-1 expression in the epithelial cells. Bafilomycin A(1)
may therefore modulate airway inflammation after RV infection.