Study of nitroxide-linked naphthalene as a fluorescence probe for hydroxylradicals

A novel technique for the determination of hydroxyl radical is described. T he method employed the reaction of hydroxyl radical with dimethyl sulfoxide quantitatively to generate a methyl radical, which then coupled with nitro xide-linked naphthalene and led to a large increase in fluorescence intensi ty. The fluorescence increment was proportional to the concentration of hyd roxyl radicals down to 1 x 10(-7) mol l(-1). The proposed method was demons trated to be capable of detecting hydroxyl radicals generated in Fenton sys tems. It proved to be a simple and sensitive technique for the determinatio n of hydroxyl radicals. (C) 2001 Elsevier Science B.V. All rights reserved.