Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine

Endocrine disrupting chemicals (EDCs) such as dioxins, PCBs and pesticides are very harmful to human. In this work, a rapid detection method for 2,3,7 ,8-TCDD, PCB, and atrazine is described, which is based upon a competitive immunoassay using surface plasmon resonance (SPR). With this method, it was possible to detect 2,3,7,8-TCDD, PCB, and atrazine with respective detecti on limits of 0.1, 2.5, and 5 ng ml(-1), requiring 15 min for a single sampl e measurement. To achieve better sensitivities, a sandwich assay was additi onally performed. For dioxins and atrazine, response amplification could be observed, enabling better differentiation of small concentration differenc es among analytes. (C) 2001 Elsevier Science B.V. All rights reserved.