The extracellular matrix regulates many fundamental cellular processes such
as proliferation, migration, and differentiation. Among the ECM components
, type I collagen induces endothelial tube formation in vitro. By analysing
genes participating in this event, the hone morphogenetic protein receptor
-II (BMPR-II) was detected to be upregulated in cells cultured on or within
fibrillar type I collagen. Furthermore, the basement membrane type IV coll
agen or amorphous type I collagen did not show an induction of BMPR-II, Add
ition of the BMPR-II specific ligands, BMP2 and BMP4, in the culture medium
of the endothelial cells seeded on type I collagen increased [H-3]-thymidi
ne incorporation into cellular DNA, indicating that endothelial cells were
able to form a functional receptor. In addition, in the chick chorioallanto
ic membrane (CAM), an in vivo angiogenesis model, BMPR-II and BMPR-I were u
pregulated in the growing phase and ceased in the mature CAM. (C) 2001 Acad
emic Press.