Negative regulation of YY1 transcription factor on the dynamin I gene promoter

Dynamin I is highly expressed in brain and plays a critical role in clathri n-mediated endocytosis and synaptic vesicle recycling. To elucidate the mol ecular mechanism by which expression of dynamin I is tissue-specifically re gulated, we previously cloned and characterized the promoter of the mouse d ynamin I gene and suggested that there is a negative regulatory element in this promoter region. In the present study, we showed that YY1 binds to thi s negative regulatory element located at -111 to -107 by using the EMSA and supershift analyses. Cotransfection experiment using an YY1 expression vec tor revealed that YY1 exerts a repressive role on the dynamin I gene promot er activity. These results demonstrate that transcription factor YY1 negati vely regulates dynamin I expression via binding to the negative regulatory element. (C) 2001 Academic Press.