F. Valverde et al., Simultaneous occurrence of two different glyceraldehyde-3-phosphate dehydrogenases in heterocystous N-2-fixing cyanobacteria, BIOC BIOP R, 283(2), 2001, pp. 356-363
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Enzyme activity determinations and Western and Northern blot analyses have
shown the presence of two catalytically different glyceraldehyde-3-phosphat
e dehydrogenases (GAPDH) in both vegetative cells and heterocysts of severa
l N-2-fixing Anabaena strains: (a) the gap2-encoded NAD(P)-dependent GAPDH2
(EC 1.2.1.59), the enzyme involved in the photosynthetic carbon assimilati
on pathway, which is present at higher levels in vegetative cells, and (b)
the gap3-encoded NAD-dependent GAPDH3 (EC 1.2.1.12), presumably involved in
carbohydrate anabolism and catabolism, which is the predominant GAPDH in h
eterocysts, In contrast, the gap1-encoded GAPDH1, which is the other NAD-de
pendent cyanobacterial GAPDH, is virtually absent in both cell types. These
findings are discussed in the context of carbon metabolism of heterocystou
s N-2-fixing cyanobacteria, (C) 2001 Academic Press.