High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium

Authors
Chen, ZL Lutkemeyer, D Iding, K Lehmann, J
Citation
Zl. Chen et al., High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium, BIOTECH LET, 23(10), 2001, pp. 767-770
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
10
Year of publication
2001
Pages
767 - 770
Database
ISI
SICI code
0141-5492(200105)23:10<767:HCORCH>2.0.ZU;2-#
Abstract
A recombinant CHO cell line, CHO2DS, was immobilized on porous microcarrier Cytopore 1 and cultivated in 1 l modified Super-spinner and 2 l stirred ta nk bioreactor with the perfusion of a low-cost chemically defined protein-f ree medium DF6S. CHO2DS cells could enter into the inner space and grew bot h in the inner space and on the surface of Cytopore 1 in DF6S and produced prothrombin at 22 mg l(-1) after 10 days. From a seeding density of 5.7 x 1 0(5) cells ml(-)1, the highest viable cell density of CHO2DS was 1.12 x 10( 7) cells ml(-1).