Improved conditions for the transformation by electroporation of the extracellular polysaccharide-producing methylotroph Methylobacillus sp.

Authors
Yoshida, T Ayabe, Y Horinouchi, M Habe, H Nojiri, H Omori, T
Citation
T. Yoshida et al., Improved conditions for the transformation by electroporation of the extracellular polysaccharide-producing methylotroph Methylobacillus sp., BIOTECH LET, 23(10), 2001, pp. 787-791
Citations number
19
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
10
Year of publication
2001
Pages
787 - 791
Database
ISI
SICI code
0141-5492(200105)23:10<787:ICFTTB>2.0.ZU;2-X
Abstract
The transformation efficiency of Methylobacillus sp. strain 12S, using elec troporation, was unaffected by the growth phase of the cells but competent cells grown at 21 degreesC had a 1.9 x 10(3) times higher transformation ef ficiency than those grown at 30 degreesC. Heat shock treatment further incr eased the transformation efficiency up to 7 times.