Physical stabilization of DNA-based therapeutics

The development of non-viral vectors for gene delivery has primarily focuse d on improving the efficiency of gene transfer in vivo. Although there is c learly a need to improve delivery efficiency, studies also indicate that th e physical stability of non-viral vectors is not nearly adequate for a mark etable pharmaceutical product. Here, we describe the different strategies t hat have been used to enhance stability and discuss the mechanisms by which prolonged stabilization (>2 years) might be achieved.