A UNIVERSAL PROCEDURE FOR PRIMER LABELING OF AMPLICONS

Detection and visualisation of nucleic acids is integral to genome ana lyses. Exponential amplification procedures have provided the means fo r the manipulation of nucleic acid sequences, which were otherwise ina ccessible. We describe the development and application of a universal method for the labelling of any PCR product using a single end-labelle d primer. Amplification was performed in a single reaction with the re sulting amplicon labelled to a high specific activity. The method was adapted to a wide range of PCRs and significantly reduced the expense of such analyses.