Evaluation of ODS-AQ stationary phase for use in capillary electrochromatography

The aim of this study was to evaluate the applicability of ODS-AQ packing m aterial as a stationary phase in capillary electrochromatography (CEC). The electroosmotic flow created on an ODS-AQ stationary phase was measured at different mobile phase compositions and at different column temperatures. i t was observed that the electroosmotic now generated in the column increase d by 50% when the temperature of the system was raised from 20 degreesC to 60 degreesC, while all other conditions were kept constant. The electroosmo tic flow produced by the ODS-AQ stationary phase was found to be comparable to the flow generated in a column packed with Nucleosil bare-silica materi al. In addition, a set of polar compounds (D-lysergic acid diethylamide der ivatives) was utilized to determine the influence of temperature and mobile phase composition on their chromatographic behavior on an ODS-AQ stationar y phase in a CEC mode. A linear relationship between the solute retention f actor and column temperatures was seen over the temperature range studied ( 20 degreesC to 60 degreesC). A quadratic function was used to describe the changes in the solute retention factors with variation of acetonitrile conc entration in the mobile phase.