Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Citation
Z. Liu et al., Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography, ELECTROPHOR, 22(7), 2001, pp. 1298-1304
Citations number
35
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
01730835 → ACNP
Volume
22
Issue
7
Year of publication
2001
Pages
1298 - 1304
Database
ISI
SICI code
0173-0835(200104)22:7<1298:SO4>2.0.ZU;2-F
Abstract
4-Dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) is a novel derivatizing reagent specially designed for the determination o f carbonyl compounds. In this work, we describe the separation of DMNTH-der ivatized carbonyl compounds by reversed-phase capillary electrochromatograp hy (CEC). After systematic investigations of the effects of experimental co nditions viz. pH and concentration of buffer, type of stationary phase, inj ection volume of sample, organic modifier, and temperature, optimal conditi ons were found. The sample compounds, which were separated with gradient hi gh performance liquid chromatography (HPLC), were separated by CEC under is ocratic elution due to the high efficiency. Comparisons of separations by C EC and micellar electrokinetic chromatography (MEKC) were made.