Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

4-Dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) is a novel derivatizing reagent specially designed for the determination o f carbonyl compounds. In this work, we describe the separation of DMNTH-der ivatized carbonyl compounds by reversed-phase capillary electrochromatograp hy (CEC). After systematic investigations of the effects of experimental co nditions viz. pH and concentration of buffer, type of stationary phase, inj ection volume of sample, organic modifier, and temperature, optimal conditi ons were found. The sample compounds, which were separated with gradient hi gh performance liquid chromatography (HPLC), were separated by CEC under is ocratic elution due to the high efficiency. Comparisons of separations by C EC and micellar electrokinetic chromatography (MEKC) were made.