Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types

A truncated form of the structural protein VP2 (truncVP2) of infectious pan creatic necrosis (IPN) virus encompassing amino acids 147-307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant ant igens were recognised by a VP2-specific monoclonal antibody by ELISA and im munoblot analysis. However, the minimum amount of r-truncVP2 needed for det ection by these methods varies depending on the cell type used for expressi on. Furthermore, all four recombinant preparations, when used to immunise A tlantic salmon, were capable of inducing antibodies reactive with whole IPN V in ELISA. (C) 2001 Academic Press.