NMR spin trapping: Detection of free radical reactions with a new fluorinated DMPO analog

Citation
Vv. Khramtsov et al., NMR spin trapping: Detection of free radical reactions with a new fluorinated DMPO analog, FREE RAD B, 30(10), 2001, pp. 1099-1107
Citations number
15
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN journal
08915849 → ACNP
Volume
30
Issue
10
Year of publication
2001
Pages
1099 - 1107
Database
ISI
SICI code
0891-5849(20010515)30:10<1099:NSTDOF>2.0.ZU;2-O
Abstract
Electron spin resonance (ESR) and nuclear magnetic resonance (NMR) spin tra pping were used for detection of free radical reactions utilizing a new flu orinated analog of DMPO, 4-hydroxy-5,5-dimethyl-2-trifluoromethylpyrroline- 1-oxide (FDMPO). The parent FDMPO spin trap exhibits a single F-19-NMR reso nance at -66.0 ppm. The signal to noise ratio improved 10.4-fold compared t o P-31-NMR sensitivity of the phosphorus-containing spin trap, DEPMPO. The spin adducts of FDMPO with (OH)-O-., (CH3)-C-., and (CH2OH)-C-. were charac terized. Competitive spin trapping of FDMPO with DMPO showed that both have similar rates of addition of (OH)-O-. and C-centered radicals. The corresp onding paramagnetic spin adducts of FDMPO were extremely stable to degradat ion. In the presence of ascorbate, reaction products from C-centered radica ls resulted in the appearance of two additional F-19-NMR signals at -78.6 a nd -80 ppm for FDMPO/(CH3)-C-. and at -74.6 and -76.75 ppm for FDMPO/(CH2OH )-C-.. In each case, these peaks were assigned to the two stereoisomers of their respective, reduced hydroxylamines. The identification of the hydroxy lamines for FDMPO/(CH3)-C-. was confirmed by EPR and F-19-NMR spectra of in dependently synthesized samples. In summary, spin adducts of FDMPO were hig hly stable for ESR. For NMR spin trapping, FDMPO showed improved signal to noise and similar spin trapping efficiency compared to DEPMPO. (C) 2001 Els evier Science Inc.