Rp1 is a complex rust resistance locus of maize. The HRp1-D haplotype is co
mposed of Rp1-D and eight paralogues, seven of which also code fur predicte
d nucleotide binding site-leucine rich repeat (NBS-LRR) proteins similar to
the Rp1-D gene. The paralogues are polymorphic (DNA identities 91-97%), es
pecially in the C-terminal LRR domain. The remaining family member encodes
a truncated protein that has no LRR domain. Seven of the nine family member
s, including the truncated gene, are transcribed. Sequence comparisons betw
een paralogues provide evidence for past recombination events between paral
ogues and diversifying selection, particularly in the C-terminal half of ti
le LRR domain. Variants selected for complete or partial loss of Rp1-D resi
stance can he explained by unequal crossing over that occurred mostly withi
n coding regions. The Rp1-D gene is altered or lost in all variants, the re
combination breakpoints occur throughout the genes, and most recombinant ev
ents (9/14 examined) involved the same untranscribed paralogue with the Rp1
-D gene. One recombinant with a complete LRR from Rp1-D, hut the aminotermi
nal portion from another homologue, conferred the Rp1-D specificity but wit
h a reduced level of resistance.