Polyamine enhancement of the synthesis of adenylate cyclase at the translational level and the consequential stimulation of the synthesis of the RNA polymerase sigma(28) subunit

Citation
M. Yoshida et al., Polyamine enhancement of the synthesis of adenylate cyclase at the translational level and the consequential stimulation of the synthesis of the RNA polymerase sigma(28) subunit, J BIOL CHEM, 276(19), 2001, pp. 16289-16295
Citations number
62
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
19
Year of publication
2001
Pages
16289 - 16295
Database
ISI
SICI code
0021-9258(20010511)276:19<16289:PEOTSO>2.0.ZU;2-E
Abstract
The effects of polyamines on the synthesis of various a subunits of RNA pol ymerase were studied using Western blot analysis. Synthesis of sigma (28) w as stimulated 4.0-fold and that of sigma (38) was stimulated 2.3-fold by po lyamines, whereas synthesis of other a subunits was not influenced by polya mines. Stimulation of sigma (28) synthesis was due to an increase in the le vel of cAMP, which occurred through polyamine stimulation of the synthesis of adenylate cyclase at the level of translation. Polyamines were found to increase the translation of adenylate cyclase mRNA by facilitating the UUG codon-dependent initiation. Analysis of RNA secondary structure suggests th at exposure of the Shine-Dalgarno sequence of mRNA is a prerequisite for po lyamine stimulation of the UUG codon-dependent initiation.