Measurement of homonuclear three-bond J((HH alpha)-H-N) coupling constantsin unlabeled peptides complexed with labeled proteins: Application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV)

Authors
Cicero, DO Barbato, G Koch, U Ingallinella, P Bianchi, E Sambucini, S Neddermann, P De Francesco, R Pessi, A Bazzo, R
Citation
Do. Cicero et al., Measurement of homonuclear three-bond J((HH alpha)-H-N) coupling constantsin unlabeled peptides complexed with labeled proteins: Application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV), J BIOM NMR, 20(1), 2001, pp. 23-29
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOMOLECULAR NMR
ISSN journal
09252738 → ACNP
Volume
20
Issue
1
Year of publication
2001
Pages
23 - 29
Database
ISI
SICI code
0925-2738(200105)20:1<23:MOHTJA>2.0.ZU;2-G
Abstract
A new isotope-filtered experiment has been designed to measure homonuclear three-bond J((HHalpha)-H-N) coupling constants of unlabeled peptides comple xed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the 'quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS 3 protein of human hepatitis C virus (HCV).