Vj. Warburton-timms et Cam. Mcnulty, Role of screening agar plates for in vitro susceptibility testing of Helicobacter pylori in a routine laboratory setting, J CLIN PATH, 54(5), 2001, pp. 408-411
Citations number
12
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Background-Resistance of Helicobacter pylori to the more frequently used an
tibiotics (metronidazole and clarithromycin) reduces eradication rates even
with triple treatment. Determining the antibiogram profile of H pylori can
take up to 14 days and delays appropriate treatment.
Aims-To determine the role of screening agar plates for more rapid in vitro
susceptibility of H pylori to metronidazole, amoxicillin, and clarithromyc
in.
Methods-Routine gastric biopsy specimens from 507 dyspeptic patients were i
noculated on to 10% lysed blood agar plates containing metronidazole (8 mug
/ mi), clarithromycin (2 mug/ml), or amoxicillin (0.5 mug/ml). The minimum
inhibitory concentration (MIC) of the 90 isolates was determined using the
E test.
Results-Metronidazole resistance was detected in 28 of 90 isolates by E tes
t and nine of 98 by screening agar. The screening agar detected none of the
four clarithromycin resistant isolates detected by the E test.
Conclusions-The screening agar method is not sufficiently sensitive to be u
sed alone.