An endoplasmic reticulum retention function for the cytoplasmic tail of the human pre-T cell receptor (TCR) alpha chain: Potential role in the regulation of cell surface pre-TCR expression levels

The pre-T cell receptor (TCR), which consists of a TCR-beta chain paired wi th pre-TCR-alpha (pT alpha) and associated with CD3/zeta components, is a c ritical regulator of T cell development. For unknown reasons, extremely low pre-TCR levels reach the plasma membrane of pre-T cells, By transfecting c himeric TCR-alpha -pT alpha proteins into pre-T and mature T cell lines, we show here that the low surface expression of the human pre-TCR is pT alpha chain dependent. Particularly, the cytoplasmic domain of pT alpha is suffi cient to reduce surface expression of a conventional TCR-alpha/beta to pre- TCR expression levels. Such reduced expression cannot be attributed to qual itative differences in the biochemical composition of the CD3/zeta modules associated with pre-TCR and TCR surface complexes, Rather, evidence is prov ided that the pT alpha cytoplasmic tail also causes a reduced surface expre ssion of individual membrane molecules such as CD25 and CD4, which are show n to be retained in the endoplasmic reticulum (ER). Native pT alpha is also observed to be predominantly ER localized. Finally, sequential truncations along the pT alpha cytoplasmic domain revealed that removal of the COOH-te rminal 48 residues is sufficient to release a CD-4-pT alpha chimera from ER retention, and to restore native CD4 surface expression levels. As such a truncation in pT alpha also correlates with enhanced pre-TCR expression. th e observed pT alpha ER retention function may contribute to the regulation of surface pre-TCR expression on pre-T cells.