Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis,
circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only
mononuclear complexes of stoichiometries ranging from CuL to CuH-3L were f
ound. In the main species present at neutral pH, CuH-2L, lincomycin bonds C
u(II) through both of its nitrogen donors, and a deprotonated oxygen donor
at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of pr
oducts of 2'-deoxyguanosine (dc) oxidation and agarose gel electrophoresis
of plasmid DNA confirmed that lincomycin complexes effectively facilitate d
G oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA
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