Copper(II)-lincomycin: complexation pattern and oxidative activity

Citation
M. Jezowska-bojczuk et al., Copper(II)-lincomycin: complexation pattern and oxidative activity, J INORG BIO, 84(3-4), 2001, pp. 189-200
Citations number
47
Categorie Soggetti
Biochemistry & Biophysics","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN journal
01620134 → ACNP
Volume
84
Issue
3-4
Year of publication
2001
Pages
189 - 200
Database
ISI
SICI code
0162-0134(200104)84:3-4<189:CCPAOA>2.0.ZU;2-I
Abstract
Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH-3L were f ound. In the main species present at neutral pH, CuH-2L, lincomycin bonds C u(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of pr oducts of 2'-deoxyguanosine (dc) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate d G oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA . (C) 2001 Elsevier Science B.V. All rights reserved.