Purpose: Trauma, operations or instrumentation of the urethra or ureter may
lead to stricture disease. The use of a natural urethral stent made of aut
ologous tissue would be advantageous due to its biocompatibility. In this s
tudy we investigated the feasibility of engineering cartilage stents in vit
ro and in vivo.
Materials and Methods: We fabricated 40 cylinders 10 mm. long with an inner
and outer diameter of 5 and 9 mm., respectively, from polyglycolic acid me
sh coated with 50:50 polylactic-co-glycolic acid. Chondrocytes isolated fro
m bovine shoulders were seeded onto the tubular polymer scaffolds at a seed
ing density of 60 x 10(6) cells per mi. Scanning electron microscopy was pe
rformed to determine the even distribution of chondrocytes throughout the p
olymer scaffolds. We implanted 20 cylinders under the skin of nude mice and
20 were cultured in stirred bio-reactors. Cytological characteristics, col
lagen content and mechanical durability were evaluated 4 and 10 weeks after
cell seeding.
Results: Gross examination of the engineered stents showed the solid, glist
ening appearance of cartilaginous tissue. Cytological analyses with hematox
ylin and eosin, trichrome, alcian blue and safranin O confirmed cartilage,
and the deposition of collagen and glycosaminoglycan in each group. Increas
ed deposition of collagen and glycosaminoglycan was observed in the stents
created in vivo. Biomechanical testing demonstrated that the cartilaginous
cylinders in each group were readily elastic and withstood high degrees of
pressure.
Conclusions: This study demonstrates the feasibility of creating cartilagin
ous stents in vitro and in vivo using chondrocyte seeded polymer matrices.
This technology may be useful clinically for stricture disease in the genit
ourinary tract.