Immunoglobulin G (IgG) and IgA, but also nonantibody factors, account for in vitro neutralization of human immunodeficiency virus (HIV) type 1 primary isolates by serum and plasma of HIV-infected patients

Authors
Burrer, R Salmon-Ceron, D Richert, S Pancino, G Spiridon, G Haessig, S Roques, V Barre-Sinoussi, F Aubertin, AM Moog, C
Citation
R. Burrer et al., Immunoglobulin G (IgG) and IgA, but also nonantibody factors, account for in vitro neutralization of human immunodeficiency virus (HIV) type 1 primary isolates by serum and plasma of HIV-infected patients, J VIROLOGY, 75(11), 2001, pp. 5421-5424
Citations number
19
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
11
Year of publication
2001
Pages
5421 - 5424
Database
ISI
SICI code
0022-538X(200106)75:11<5421:IG(AIB>2.0.ZU;2-E
Abstract
The factors present in serum and plasma samples of human immunodeficiency v irus (HIV)-infected patients that are responsible for the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro have been analyzed. Puri fication of immunoglobulins (Ig) by affinity chromatography showed that the activities were mostly attributable to Ige and less frequently to IgA, For two samples, we have shown that the high-level and broad-spectrum inhibito ry activity was essentially caused by non-Ig factors interfering with the m easurement of antibody-specific neutralizing activity.