Dj. Mckenna et al., Advanced glycation alters expression of the 67kDa laminin receptor in retinal microvascular endothelial cells, LIFE SCI, 68(24), 2001, pp. 2695-2703
The 67kDa laminin receptor (67LR) plays an important role in vascular cell
function and dysfunction. The present study has examined 67LR expression in
retinal microvascular endothelial cells after exposure to AGEs. Retinal mi
crovascular endothelial cells were exposed to either AGE-BSA, or were grown
on methylglyoxal-modified laminin or Matrigel (TM) and expression of 67LR
analysed by Western Blotting and RT-PCR/Southern blotting. Western blotting
of plasma membrane and RT-PCR/Southern blotting revealed a significant upr
egulation of 67LR protein/mRNA expression after exposure to AGEs (p <0.05-0
.01). The results show that 67LR is upregulated in cells exposed to AGEs an
d suggests a previously unrecognised role for this receptor in retinal micr
ovascular endothelial cell interaction with glycated substrates. (C) 2001 E
lsevier Science Inc. All rights reserved.