A simple, reliable, and fast protocol for thraustochytrid DNA extraction

dDNA extraction of thraustochytrids, common marine unicellular organisms, i s usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytri d total DNA extraction is described. The average isolated total DNA is 20 t o 40 kb, and DNA samples are suitable for a variety of uses including 18S-r ibosomal UNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also f aster than the other protocols.