Interaction of pea (Pisum sativum L.) lectins with rhizobial strains

Authors
Bajaj, M Soni, G Singh, CK
Citation
M. Bajaj et al., Interaction of pea (Pisum sativum L.) lectins with rhizobial strains, MICROBI RES, 156(1), 2001, pp. 71-74
Citations number
20
Categorie Soggetti
Biology
Journal title
MICROBIOLOGICAL RESEARCH
ISSN journal
09445013 → ACNP
Volume
156
Issue
1
Year of publication
2001
Pages
71 - 74
Database
ISI
SICI code
0944-5013(2001)156:1<71:IOP(SL>2.0.ZU;2-Y
Abstract
Lectins from two varieties (PG-3 and LFP-48) of pea have been purified by a ffinity chromatography on Sephadex G-50. The specific activity increased by 23 and 25 folds, respectively. These lectins from both the varieties were found to be specific for mannose. The purified fluorescein isothiocyanate ( FITC)- labelled lectins showed binding reaction with homologous as well as heterologous strains of Rhizobium spp. The results revealed that pea lectin s are not highly specific to their respective rhizobia. Moreover, these lec tins showed a greater stimulatory effect on homologous Rhizobium leguminosa rum strains.