5-aminosalicylic acid protection against oxidative damage to synaptosomal membranes by alkoxyl radicals in vitro

The antioxidant properties of 5-aminosalicylic acid in vitro were evaluated in a synaptosomal membrane system prepared from gerbil cortical synaptosom es using EPR spin labeling and spectroscopic techniques. MAL-6 (2,2.6,6-tet ramethyl-4-maleimidopiperidin-1-oxyl) and 5-NS (5-nitroxide stearate) spin labels were used to assess changes in protein oxidation and membrane lipid fluidity, respectively. Synaptosomal membranes were subjected to oxidative stress by incubation with 1 mM azo-bis(isobutyronitrile) (AIBN) or 1 mM 2,2 '-azobis(amidino propane) dihydrochloride (AAPH) at 37 degreesC for 30 minu tes. The EPR analyses of the samples showed significant oxidation of synapt osomal proteins and a decrease in membrane fluidity. 5-Aminosalicylic acid also was evaluated by means of FRAP (the ferric reducing ability of plasma) test as a potential antioxidant. 5-Aminosalicylic acid also showed protect ion against the oxidation in gerbil cortical synaptosomes system caused by AIBN and AAPH. These results are consistent with the notion of antioxidant protection against free radical induced oxidative stress in synaptosomal me mbrane system by this agent.