Two glnB-like genes have been isolated from Herbaspirillum seropedicae
by complementation of the Klebsiella pneumoniae glnB502 mutant for gr
owth on nitrate. One of these glnB-like genes has been sequenced and s
hows strong identity with GlnB proteins derived from other organisms.
A Tn5-20 mutation of this glnB was Nif negative.