Localization of proteins immunologically related to subunits of stress 310-kD protein in winter wheat mitochondria

Authors
Pobezhimova, TP Grabel'nykh, OI Kolesnichenko, AV Sumina, ON Voinikov, VK
Citation
Tp. Pobezhimova et al., Localization of proteins immunologically related to subunits of stress 310-kD protein in winter wheat mitochondria, RUSS J PL P, 48(2), 2001, pp. 204-209
Citations number
22
Categorie Soggetti
Plant Sciences
Journal title
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
ISSN journal
10214437 → ACNP
Volume
48
Issue
2
Year of publication
2001
Pages
204 - 209
Database
ISI
SICI code
1021-4437(200103/04)48:2<204:LOPIRT>2.0.ZU;2-F
Abstract
We studied the localization of polypeptides immunochemically related to sub units of cold-shock 310-kD protein from winter rye (Secale cereale L.) in m itochondria and submitochondrial structures of winter wheat (Triticum aesti vum? L.) seedlings. Polypeptides were separated by SDS-PAGE and probed with the antibody against 310-kD protein from rye seedlings. Wheat mitochondria contained the following polypeptides cross-reacting with this antibody: 66 , 60, 55, and 23 kD in the inner membrane; 60 and 58 kD in the outer membra ne; and 66 and 55 kD in the matrix.